Regulatory

Part:BBa_K2782001

Designed by: Dan Zhou   Group: iGEM18_BUCT-China   (2018-08-15)


This part can express a repressor protein that binds to a fatty acid operon

The sequence-expressing repressor recognizes a specific operator (ie, the operon is the repressor binding site), and when the manipulation sequence binds to the repressor, it blocks the binding of the RNA polymerase to the promoter sequence, preventing RNA polymerase from following the DNA moves forward, preventing transcription mediated negative regulation. When a certain amount of fatty acid is present, it can bind to the repressor protein, and the binding of the repressor protein to the operon fails, and the RNA polymerase binds to the promoter sequence and moves forward along the DNA, after which the gene begins to express.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None